Alexandra Vaisman

2000- Senior Research Fellow, NICHD, NIH
1997- 2000 Research Analyst, University of North Carolina at Chapel Hill
1992-1997 Postdoctoral Research Associate, University of North Carolina at Chapel Hill
1990-1991 Research Scientist, American Cyanamid and Medinvest Company, Joint Laboratory of Organic Synthesis Moscow, USSR
1989-1990 Research Scientist, Bioprocess Laboratories, USSR Academy of Sciences, Moscow, USSR
1985-1989 Postgraduate Associate, Research Associate, Department of Colloid Chemistry, Lomonosov Institute of Fine Chemical Technology, Moscow, USSR
1989 Ph.D. Lomonosov Institute of Fine Chemical Technology, Moscow, USSR
1985 M.S. Lomonosov Institute of Fine Chemical Technology, Moscow, USSR

SELECTED PUBLICATIONS:

Martomo SA, Yang WW, Vaisman A, Maas A, Yokoi M, Hoeijmakers JH, Hanaoka F, Woodgate R, Gearhart PJ. Normal hypermutation in antibody genes from congenic mice defective for DNA polymerase iota. DNA Repair. 2006; 5:392-8.
McIlwraith MJ, Vaisman A, Liu Y, Fanning E, Woodgate R, and West SC. Human DNA Polymerase eta Promotes DNA Synthesis from Strand Invasion Intermediates (D-loops) of Homologous Recombination. Mol Cell. 2005; 20:783-92.
Vaisman A, Takasawa K, Iwai S, and Woodgate R. DNA polymerase iota-dependent translesion replication of uracil containing cyclobutane pyrimidine dimers. DNA Repair. 2005; 5:210-8.
Vaisman A, Ling H, Woodgate R, and Yang W. (2005) Fidelity of Dpo4: effect of metal ions, nucleotide selection and pyrophosphorolysis. EMBO J. 24: 2957-67.

Wilson TM, Vaisman A, Martomo SA, Sullivan P, Lan L, Hanaoka F, Yasui A, Woodgate R, and Gearhart PJ. (2005) MSH2-MSH6 stimulates DNA polymerase eta, suggesting a role for A:T mutations in immunoglobulin genes. J. Exp. Med. 201:637-45.

Vaisman A and Woodgate R. (2004) DNA Eukaryotic Translesion DNA Polymerases. Encyclopedia of Biol. Chem. 4:247-50.

Vaisman A, Lehmann AR, Woodgate R. (2004) DNA Polymerases eta and iota. Adv Protein Chem. 69:205-28.

Ghadessy FJ, Ramsay N, Boudsocq F, Loakes D, Brown A, Iwai S, Vaisman A, Woodgate R, Holliger P. (2004) Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution. Nat Biotechnol. 22:755-9

Boudsocq F, Kokoska RJ, Plosky BS, Vaisman A, Ling H, Kunkel TA, Yang W, Woodgate R. (2004) Investigating the role of the little finger domain of Y-family DNA polymerases in low fidelity synthesis and translesion replication. J Biol Chem. 279:32932-40.

Bassett E, Vaisman A, Havener JM, Masutani C, Hanaoka F, Chaney SG. (2003) Efficiency of extension of mismatched primer termini across from cisplatin and oxaliplatin adducts by human DNA polymerases beta and eta in vitro. Biochemistry,42:14197-206.

Vaisman A, Frank EG, Iwai S, Ohashi E, Ohmori H, Hanaoka F, Woodgate R. (2003) Sequence context-dependent replication of DNA templates containing UV-induced lesions by human DNA polymerase iota. DNA Repair 2:991-1006.

Bassett E, Vaisman A, Tropea KA, McCall CM, Masutani C, Hanaoka F, Chaney SG. (2002) Frameshifts and deletions during in vitro translesion synthesis past Pt-DNA adducts by DNA polymerases beta and eta. DNA Repair 1:1003-16.

Vaisman A, Frank EG, McDonald JP, Tissier A, Woodgate R. (2002) poliota-dependent lesion bypass in vitro. Mutat Res. 510: :9-22.

Vaisman A, Woodgate R. (2001) Unique misinsertion specificity of poliota may decrease the mutagenic potential of deaminated cytosines. EMBO J. 20: 6520-9.

Vaisman A, Tissier A, Frank EG, Goodman MF, Woodgate R. (2001) Human DNA polymerase iota promiscuous mismatch extension. J Biol Chem. 276:30615-22.

Tissier A, Frank EG, McDonald JP, Vaisman A, Fernandez de Henestrosa AR, Boudsocq F, McLenigan MP, Woodgate R. (2001) Biochemical characterization of human DNA polymerase iota provides clues to its biological function. Biochem Soc Trans., 29:183-7.

Vaisman A, Warren MW, Chaney SG. (2001) The effect of DNA structure on the catalytic efficiency and fidelity of human DNA polymerase b on templates with platinum-DNA adducts. J Biol Chem, 276:18999-9005.

Woynarowski JM, Faivre S, Herzig MC, Arnett B, Chapman WG, Trevino AV, Raymond E, Chaney SG, Vaisman A, Varchenko M, Juniewicz PE. (2000) Oxaliplatin-induced damage of cellular DNA. Mol Pharmacol. 58: 920-7.

Vaisman A, Chaney SG. (2000) The efficiency and fidelity of translesion synthesis past cisplatin and oxaliplatin GpG adducts by human DNA polymerase beta. J Biol Chem. 275: 13017-25.

Vaisman A, Masutani C, Hanaoka F, Chaney SG. (2000) Efficient translesion replication past oxaliplatin and cisplatin GpG adducts by human DNA polymerase eta. Biochemistry, 39: 4575-80.

Chaney S.G.and Vaisman A. (1999) Specificity of Platinum DNA Adduct Repair. Journal of Inorganic Biochemistry, 77:71-81.

Chaney S.G.,Vaisman A., DNA adduct tolerance and bypass. Platinum-Based Drugs in Cancer Therapy, Chapter 6, Humana Press, Totowa, NJ, 129-148.

Vaisman A, Lim SE, Patrick SM, Copeland WC, Hinkle DC, Turchi JJ, Chaney SG: (1999). The Effect of DNA Polymerases and HMG1 on the Carrier Ligand Specificity for Translesion Synthesis Past Pt-DNA Adducts, Biochemistry, 38:11026-11039.

Reardon JT, Vaisman A, Chaney SG, and Sancar A (1999). Efficient Nucleotide Excision Repair of Cisplatin, Oxaliplatin and JM216 Platinum Intrastrand DNA Diadducts Cancer Res., 59:3968-3971.

Vaisman, A., Varchenko, M., Umar, A., Kunkel, T.A., Risinger, J.I., Barrett, J.C., Hamilton, T.C., & Chaney, S.G. (1998). The role of hMLH1, hMSH3, and hMSH6 defects in cisplatin and oxaliplatin resistance: correlation with replicative bypass of platinum- DNA adducts. Cancer Res., 58, 3579-3585.

Vaisman, A., Varchenko, M., Said, I., & Chaney, S.G. (1997). Cell cycle changes associated with formation of Pt-DNA adducts in human ovarian carcinoma cells with different cisplatin sensitivity. Cytometry, 27, 54-64.

Delmastro, D.A., Li, J., Vaisman, A., Solle, M., & Chaney, S.G. (1997). DNA damage inducible-gene expression following platinum treatment in human ovarian carcinoma cell lines. Cancer Chemother.Pharmacol., 39, 245-253.

Vaisman, A., Keeney, S., Nichols, A.F., Linn, S., & Chaney, S.G. (1996). Cisplatin-induced alterations in the expression of the mRNAs for UV- damage recognition protein. Oncol.Res., 8, 7-12.

Shea T., Graham M., Bernard S., Steagall A., Wiley J., Serody J., Brecher M., Bentley S. Johnston C., Vaisman A., Chaney S.G., Letrent S., Brouer K. A clinical and pharmacokinetic study of high-dose carboplatin, paclitaxel, granulocyte colony-stimulating factor, and peripheral blood stem cells in patients with unresectable or metastatic cancer. Seminars in Oncology. 1995, 22, 80-5.

Vaisman, A. & Chaney, S.G. (1995). Induction of UV-damage recognition protein by cisplatin treatment. Biochemistry, 34, 105-114.

Thompson, D.C., Vaisman, A., Sakata, M.K., Wyrick, S.D., Holbrook, D.J., & Chaney, S.G. (1995). Organ-specific biotransformation of ormaplatin in the Fischer 344 rat. Cancer Chemother.Pharmacol., 36, 439-447.

Background: fragments from The Magic Carpet by Alexander Vaisman